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F8646
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
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About This Item
Empfohlene Produkte
Biologische Quelle
goat
Qualitätsniveau
Konjugat
FITC conjugate
Antikörperform
affinity isolated antibody
Antikörper-Produkttyp
secondary antibodies
Klon
polyclonal
Form
buffered aqueous solution
Lagerbedingungen
protect from light
Methode(n)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
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Allgemeine Beschreibung
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody is specific for the Fc fragment of mouse IgG. No cross reactivity is observed with the Fab fragment of mouse IgG. Purified antibody is conjugated to fluorescein isothiocyanate (FITC) in an alkaline reaction and then purified to remove unbound FITC.
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody is specific for the Fc fragment of mouse IgG. No cross reactivity is observed with the Fab fragment of mouse IgG. Purified antibody is conjugated to fluorescein isothiocyanate (FITC) in an alkaline reaction and then purified to remove unbound FITC.
Spezifität
Binds mouse IgG; does not bind other mouse Igs.
Immunogen
Purified mouse IgG
Anwendung
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody may be used for immunohistochemistry at a working dilution of 1:320 using formalin-fixed, paraffin-embedded sections of human tonsil. Antibody dilution of 1:200 was used to label sections of human brainstems and cervical spinal cords with primary antibody against human Bcl-2 protein. The antibody was also used to label erythrocytes for photon reassignment microscopy and for FACS using melanoma cells.
Sonstige Hinweise
Antibody adsorbed with bovine, equine, and human serum proteins.
Physikalische Form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Angaben zur Herstellung
Adsorbed to reduce background with bovine, equine, or human samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
dust mask type N95 (US), Eyeshields, Gloves
Analysenzertifikate (COA)
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