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Merck

F5273

Sigma-Aldrich

Monoclonal Anti-Rat Kappa & Lambda Light Chains−FITC antibody produced in mouse

clone RT-39/RL-6, purified immunoglobulin, buffered aqueous solution

Synonym(e):

Monoclonal Anti-Rat Kappa & Lambda Light Chains

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

mouse

Konjugat

FITC conjugate

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

secondary antibodies

Klon

RT-39/RL-6, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen 25 kDa

Lagerbedingungen

protect from light

Methode(n)

dot immunobinding: 1:40
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40

Isotyp

IgG1/IgG2a

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.
The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins.

Anwendung

Monoclonal Anti-Rat Kappa & Lambda Light Chains-FITC antibody produced in mouse has been used in:
  • fluorescent dot binding immunoassay (FDIBA)
  • immunocytochemistry
  • immunohistochemistry
  • flow cytometry

Biochem./physiol. Wirkung

FITC Monoclonal Anti-Rat kappa and lambda Light Chains may serve as an essential tool in the localization of light chains on rat immunoglobulins.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Analysenzertifikate (COA)

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M M Aguilar et al.
The Journal of trauma, 45(1), 14-18 (1998-07-29)
T-cell response to trauma has been assessed primarily by sampling peripheral blood lymphocytes. We hypothesized that lymphocytes residing in tissue and traveling through lymph vessels are more likely to be activated by tissue injury and hemorrhage-induced hypoperfusion. We compared peripheral

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