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F4042

Sigma-Aldrich

Monoclonal ANTI-FLAG® M5 antibody produced in mouse

clone M5, purified immunoglobulin, buffered aqueous solution

Synonym(e):

Anti-ddddk, Anti-dykddddk

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About This Item

MDL-Nummer:
MFCD00282246
UNSPSC-Code:
12352203
NACRES:
NA.32

Biologische Quelle

mouse

Qualitätsniveau

200

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

M5, monoclonal

Form

buffered aqueous solution

Aufgereinigt durch

using Protein A

Speziesreaktivität

all

Konzentration

2-5 mg/mL

Methode(n)

western blot (chemiluminescent): 10 μg/mL

Isotyp

IgG1

Immunogene Sequenz

DYKDDDDK

Versandbedingung

dry ice

Lagertemp.

−20°C

Allgemeine Beschreibung

Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Anwendung

Monoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.

Physikalische Form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)

Angaben zur Herstellung

Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.

Sonstige Hinweise

Antibody is not calcium dependent.

Rechtliche Hinweise

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

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