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Merck

F2518

Sigma-Aldrich

Human Fibronectin

from human foreskin fibroblasts, powder, suitable for cell culture

Synonym(e):

Fibronectin

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About This Item

EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352202
NACRES:
NA.75

Preise und Verfügbarkeit sind derzeit nicht verfügbar.

product name

Fibronectin, lyophilized powder, suitable for cell culture

Biologische Quelle

human foreskin fibroblasts

Qualitätsniveau

Produktlinie

BioReagent

Form

lyophilized powder

Mol-Gew.

~550 kDa

Verpackung

pkg of 0.5 mg

Methode(n)

cell culture | mammalian: suitable

Oberflächendeckung

1‑5 μg/cm2

Verunreinigungen

Human Source, none detected (HCV)
Human Source, none detected (HIV)
Human Source, none detected (HTLV)
Human Source, none detected (HbSAg)
Microbial Contamination, passes test

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Allgemeine Beschreibung

Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35.[1] Fibronectin is mostly synthesized by fibroblasts and endothelial cells.[2]It is an adhesive glycoprotein,[3] predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.[4]

Anwendung

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells
Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay[5][6] and probiglycan and cleavage assays.[7] It has also been used in ectoderm (neuronal) differentiation[8] and in the preparation of a semi-solid growth medium.[9]

Biochem./physiol. Wirkung

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis.[1] Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis.[10] Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.[2]

Leistungsmerkmale und Vorteile

  • Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.
  • It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.
  • The starting material is tested and found negative for HIV and HBsAg.
  • The product is soluble in sterile water, yielding a clear, colorless solution.
  • It can be stored at -20°C for at least three years.

Vorsicht

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Physikalische Form

Lyophilized in CAPS buffered saline

Angaben zur Herstellung

dialyzed against chloroform

Rekonstituierung

Store reconstituted solution in working aliquots at -20°C or lower.

Hinweis zur Analyse

Protein homogeneity is evaluated by immunoelectrophoresis.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

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Dies ist ein Muster eines Analysezertifikats (COA). Die darin enthaltenen Angaben beziehen sich möglicherweise nicht auf eine kürzlich hergestellte Charge dieses bestimmten Produkts.

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Ail binding to fibronectin facilitates Yersinia pestis binding to host cells and Yop delivery.
Tsang T M, et al.
Infection and Immunity, 78(8), 3358-3368 (2010)
Regulation of matrix Accumulation (2012)
Caroline Chagnot et al.
PloS one, 8(3), e59386-e59386 (2013-03-22)
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 are responsible for repeated food-poisoning cases often caused by contaminated burgers. EHEC infection is predominantly a pediatric illness, which can lead to life-threatening diseases. Ruminants are the main natural reservoir for EHEC and food contamination
Natural variant of collagen-like protein A in serotype M3 group A Streptococcus increases adherence and decreases invasive potential.
Flores A R, et al.
Infection and Immunity (2015)
Fibronectin binds and enhances the activity of bone morphogenetic protein 1.
Huang G, et al.
The Journal of Biological Chemistry (2009)

Artikel

Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protokolle

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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