E3403
EZview™ Red Protein G Affinity Gel
Synonym(e):
EZview Affinity Gel, Protein G Affinity Gel
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About This Item
Empfohlene Produkte
Form
liquid
Qualitätsniveau
chemische Klasse(n) des Analyten
proteins (Immunoglobulins of various mammalian species)
Methode(n)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
Kapazität
≥8 mg/mL, gel binding capacity (rabbit IgG)
Lagertemp.
−20°C
Allgemeine Beschreibung
EZ view Red Protein G Affinity Gel is Protein G covalently bonded to 4% Agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue, for purification of proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.
Anwendung
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Leistungsmerkmale und Vorteile
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Physikalische Form
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
50% slurry in PBS with 50%Glycerol and 0.0015%
Rechtliche Hinweise
EZview is a trademark of Sigma-Aldrich Co. LLC
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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