DUO92106

Duolink^® In Situ Orange Starter Kit Goat/Rabbit

• Synonym(e): in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit
• UNSPSC-Code: 41116158
• NACRES: NA.32

Eigenschaften

• Produktlinie: Duolink^®
• Qualitätsniveau: 100
• Methode(n): proximity ligation assay: suitable
• Fluoreszenz: λ_ex 554 nm; λ_em 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)
• Eignung: suitable for fluorescence
• Lagertemp.: −20°C

Beschreibung

Anwendung

Duolink^®proximity ligation assay(PLA^®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink^® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink^® PLA Resource Center for information on how to run a Duolink^® experiment, applications, troubleshooting, and more.

To perform a complete Duolink^® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink^® PLA reactions, which include a pair of PLA probes (Anti-Goat PLUS and Anti-Rabbit MINUS), orange detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
The Duolink^® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^® projects

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Leistungsmerkmale und Vorteile

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

Rechtliche Hinweise

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany

PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit-Komponenten auch einzeln erhältlich

• DUO92005 Duolink^® In Situ PLA^® Probe Anti-Rabbit MINUS
• DUO92003 Duolink^® In Situ PLA^® Probe Anti-Goat PLUS
• DUO92007 Duolink^® In Situ Detection Reagents Orange
• DUO82049 Duolink^® In Situ Wash Buffers, Fluorescence
• DUO82040 Duolink^® In Situ Mounting Medium with DAPI

Sicherheitshinweise

• Piktogramme: GHS08,GHS05
• Signalwort: Danger
• H-Sätze: H290,H334,H412
• P-Sätze: P234 - P261 - P273 - P284 - P390 - P501
• Gefahreneinstufungen: Aquatic Chronic 3 - Met. Corr. 1 - Resp. Sens. 1
• Lagerklassenschlüssel: 8A - Combustible corrosive hazardous materials
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable