D7069

DAPK1 (1-363), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): DKFZp7811035
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf9 cells
• Qualitätsniveau: 200
• Produktlinie: PRECISIO^® Kinase
• Assay: ≥70% (SDS-PAGE)
• Form: buffered aqueous glycerol solution
• Spezifische Aktivität: 42-57 nmol/min·mg
• Mol-Gew.: ~71 kDa
• UniProt-Hinterlegungsnummer: P53355
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... DAPK1(1612)

Beschreibung

Biochem./physiol. Wirkung

Death-associated protein kinase 1 (DAPK1) is a positive mediator of apoptosis induced by g-interferon. Activation of DAPK occurs via dephosphorylation of Ser-308 and subsequent association of calcium/calmodulin. DAPK is rapidly dephosphorylated in response to tumor necrosis factor or ceramide and then subsequently degraded via proteasome activity. The decline in DAPK expression is paralleled with increased caspase activity and cell apoptosis. Studies suggest that the apoptosis regulatory activities mediated by DAPK are controlled both by phosphorylation status and protein stability.

Physikalische Form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)