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D5042

Sigma-Aldrich

123 bp DNA Ladder

for DNA electrophoresis

Synonym(e):

DNA Ladder for DNA electrophoresis, DNA marker

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About This Item

UNSPSC-Code:
41105335
NACRES:
NA.25

Qualitätsniveau

100
200

Form

liquid

Verwendung

100 uses (0.1 mg size)
250 uses (250 μg size)

Eignung

suitable for electrophoresis (DNA)

Lagertemp.

−20°C

Anwendung

123 bp DNA Ladder has been used as a size marker in random amplified polymorphic DNA-polymerase chain reaction (PCR) analysis. It has also been used as a molecular marker in gel electrophoresis.

Komponenten

Sigma′s 123 bp DNA Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, and 0.1 mM EDTA.

Sonstige Hinweise

Contains 35 fragments, 123 - 4182 bp
For optimal resolution, the recommended agarose gel concentration is 1.5%.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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J L Hartley et al.
Gene, 13(4), 347-353 (1981-05-01)
A method for self-ligation of DNA segments, which is based on the rotational non-equivalence of ends produced by AvaI cleavage, has been developed. Using this method and an initiator molecule to encourage the formation of long polymers, we have obtained
Gene splicing and mutagenesis by PCR-driven overlap extension
Heckman KL and Pease LR
Nature Protocols, 2(4), 924-932 (2007)
E J Gubbins et al.
The Journal of biological chemistry, 255(18), 8655-8662 (1980-09-25)
The organization and sequence of the rat preprolactin gene has been investigated. Analysis of two different plasmids containing pituitary cDNA inserts has provided the complete 681-nucleotide coding sequence of preprolactin as well as 17 nucleotides preceding the initiation codon and
Sabrina Bochicchio et al.
International journal of pharmaceutics, 525(2), 377-387 (2017-02-13)
Tailored developed nanoliposomes loaded with a siRNA against the transcription factor E2F1 (siE2F1), were produced and delivered to human colorectal adenocarcinoma cell lines and to intestinal human biopsies. siE2F1 loaded nanoliposomes were produced through a dedicated ultrasound assisted technique producing

Artikel

Markers and Ladders for Gel Electrophoresis

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

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