CHP2NC
Imprint® Ultra Chromatin Immunoprecipitation Kit, Without Controls
ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing
About This Item
Empfohlene Produkte
Qualitätsniveau
Allgemeine Beschreibung
Anwendung
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Leistungsmerkmale und Vorteile
- Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
- Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 106
- Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 106 cells
- Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.
Rechtliche Hinweise
Nur Kit-Komponenten
- Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water
- Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution
Kit-Komponenten auch einzeln erhältlich
- S6576ChIP Next Gen Seq Sepharose™SDB
- I8896IGEPAL® CA-630, for molecular biologySDB
- S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell cultureSDB
- W4502Water, Nuclease-Free Water, for Molecular BiologySDB
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderSDB
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderSDB
- A7638Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)SDB
- A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)SDB
- D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridizationSDB
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionSDB
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDB
- R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)SDB
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
Zielorgane
Central nervous system
Lagerklassenschlüssel
5.1A - Strongly oxidizing hazardous materials
Analysenzertifikate (COA)
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Artikel
Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.
Protokolle
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Verwandter Inhalt
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
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