Ri-1

96090512, human blood, Lymphoblastoid

• Synonym(e): Ri-1 Cell Line
• UNSPSC-Code: 41106514

Eigenschaften

• product name: Ri-1, 96090512
• Biologische Quelle: human blood
• Wachstumsmodus: Suspension
• Karyotyp: 92XXXX/69XXX,t(1,2)(p32,q34)t(2,8)(p11,q24)t(14,18
• Morphologie: Lymphoblastoid
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian B-cell lymphoma

Beschreibung der Zelllinie

The cell line Ri-1 has been established from the peripheral blood of a 48-year-old female patient with B-cell lymphoma. This EBV-negative cell line with 14q+ abnormality was shown to be at an intermediate stage of B-cell differentiation. Ri-1 expresses surface and cytoplasmic immunoglobulin (IgM kappa), HLA-DR, CD20, CD38 and FMC7. Rearranged heavy-light chain genes and one rearranged kappa light-chain gene was detected as well as a kappa and lambda light-chain gene in germ-line configuration.

Anwendung

Study of B-cell lymphoma-leukaemia

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cell growth is very slow after resuscitation; large amounts of debris present.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.