MOR/CPR

96042333, human lung, Not specified

• UNSPSC-Code: 41106514

Eigenschaften

• product name: MOR/CPR, 96042333
• Biologische Quelle: human lung
• Wachstumsmodus: Aggregates in suspension
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human lung adenocarcinoma, drug-resistant

Beschreibung der Zelllinie

MOR/CPR has been developed by growing the parent line, MOR, in increasing concentrations of cisplatin. The cells are cross-resistant to melphalan but show little or no cross-resistance to other platinum compounds. Cisplatin accumulation has been found to be reduced compared to the parent line.

Anwendung

Analysis of acquired drug resistance

Nährmedium

RPMI 1640 + 2mM Glutamine + 1μg/ml cisplatin + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a

Sonstige Hinweise

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