AFRC 58D6

94120827, Reactivity: Anti-Haemagglutinin, influenza

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Horse x Mouse/Horse Hybridoma
• ImmunoDon: Horse sub-mandibular lymph node
• Myelom: AFRC 17D1
• Reaktivität: Anti-Haemagglutinin, influenza
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Horse sub-mandibular lymph node x AFRC 17D1

Beschreibung der Zelllinie

The heterohybridoma AFRC 58D6 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 58D6 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Immunogen

Influenza A equine 2 virus

Anwendung

Haemagglutinin detection

The isotype of the monoclonal antibody produced by this cell line is horse IgG.

Nährmedium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Sonstige Hinweise

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