PG-4

94102703

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: feline brain
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Glial/astrocyte
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Cat brain Moloney sarcoma virus-transformed

Beschreibung der Zelllinie

PG-4 was derived at NIH in 1980 from G355 cells by transformation with Moloney Murine sarcoma virus (M-MST) and subsequently cloned. Support the growth of simian sarcoma associated virus (SSAV); macaque virus; feline immunodeficiency virus (FeLV) A, B and C; feline RD-114; C retroviruses; murine xenotropic and munk cell focus-inducing virus (MCF).

Anwendung

Cells can be used to detect and assay type C retroviruses and are currently recommended by the FDA for the detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use.

PG-4 cells can be used to detect and assay type C retroviruses and are currently recommended by the FDA for the detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use.

Nährmedium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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