SK-PN-DW

94092301, human nerve, Polygonal

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: SK-PN-DW, 94092301
• Biologische Quelle: human nerve
• Wachstumsmodus: Semi-adherent
• Karyotyp: 82XXY
• Morphologie: Polygonal
• Produkte: Pro-cholecystokinin (Pro-cck)
• Rezeptoren: Vimentin, P53, anti-MIC2
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human primitive neuroectodermal tumour

Beschreibung der Zelllinie

SK-PN-DW has been derived from a male patient with a primitive neuroectodermal tumour that expresses cholecystokinin (cck) RNA and synthesises substantial amounts of pro-cck. SK-PN-DW is able to process the pro-hormone, producing immunoreactive cck-like material plus a peptide that immunochemically and chromatographically resembles intact cck c-terminal extension peptide. Cells have a tendency to float.

Anwendung

Tumour lines should prove useful in understanding the regulation of the cck gene in human neuronal cells and will also provide an in vitro system for investigation of the post translational processing of the cck preprohormone.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 9
D16S539: 12
D5S818: 13,14
D7S820: 8,14
THO1: 6
TPOX: 8,9
vWA: 16

Nährmedium

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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