KYSE-70

94072012, human esophagus, Epithelial

• Synonym(e): KY70 Cells, KYSE70 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• product name: KYSE-70, 94072012
• Biologische Quelle: human esophagus
• Wachstumsmodus: Adherent
• Karyotyp: Hypotriploid with 8% polyploidy
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human oesophageal squamous cell carcinoma

Beschreibung der Zelllinie

Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time ~35 hours.

Sonstige Hinweise

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