DH82

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94062922, dog blood, Macrophage

• Synonym(e): DH82 Cell Line
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: dog blood
• Verpackung: tube of 5 μg 94062922-DNA-5UG; pkg of vial of cells 94062922-1VL
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Macrophage
• Produkte: Not specified
• Rezeptoren: FC-gamma receptors
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Canine Monocyte-macrophage

Beschreibung der Zelllinie

Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1.

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subkultur-Routine

Maintain cultures at 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells are prone to sticking and can be removed with 0.02% EDTA.

Sonstige Hinweise

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