RC-4B/C1

94020810, rat brain, Epithelial-like

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: RC-4B/C1, 94020810
• Biologische Quelle: rat brain
• Verpackung: tube of 5 μg 94020810-DNA-5UG; pkg of vial of cells 94020810-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial-like
• Produkte: Gonadatrophs
• Rezeptoren: Gonadotrophin-releasing hormone receptors
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat pituitary adenoma

Beschreibung der Zelllinie

RC-4B/C1 is a clone of the parent RC-4B/C which was established from a 3 year-old CAI/FMaixF344/FMai(F1) male rat pituitary adenoma. Staining studies have shown the cell line contains many bi-hormonal cells with FHSß, LHß and prolactin. Electron microscopy shows the appearance of endogenous C-type rat retroviral particles.

Anwendung

Study of gonadotrophic functions of pituitary gland and regulation of pituitary gonadotrophin gene expression.

Nährmedium

DMEM:EMEM (EBSS) (1:1) + 2mM Glutamine + 0.2mg/ml BSA + 0.5% Non Essential Amino Acids (NEAA) + 5% Nu-Serum (Collab)+ 2ng/ml EGF.

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:6 i.e. seeding at 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 10% CO₂; 34°C. Saturation density is reached at 1x 100,000 cells/cm². Cells may attach lightly and so handling should be kept to a minimum. Care shoul

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