BE10-7

93040125, rat brain, Not specified

• UNSPSC-Code: 41106514
• Biologische Quelle: rat brain
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Not specified
• Rezeptoren: Not specified
• Relevante Krankheit(en): cancer

Eigenschaften

• Produktbezeichnung: BE10-7, 93040125
• Biologische Quelle: rat brain
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat, BDIX, brain, pre-malignant

Beschreibung der Zelllinie

Clone of the line BE10-Intermediate (Sigma Catalogue number. 93040119). The cells have elevated levels of tissue plasminogen activity but do not grow in agar or in BDIX rats. The cells should not be used beyond passage 24 as they start to form tumours.

Anwendung

Study of carcinogenesis

Nährmedium

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm² using 0.25% trypsin/EDTA; 10-15% CO₂; 37°C.

Sonstige Hinweise

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