70Z/3

91092002, mouse blood, Lymphoblastoid

• UNSPSC-Code: 41106514
• Biologische Quelle: mouse blood
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblastoid
• Produkte: Cytoplasmic Immunoglobulin
• Rezeptoren: Not specified
• Relevante Krankheit(en): cancer

Eigenschaften

• Produktbezeichnung: 70Z/3, 91092002
• Biologische Quelle: mouse blood
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblastoid
• Produkte: Cytoplasmic Immunoglobulin
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse pre-B lymphocyte

Beschreibung der Zelllinie

Derived from a methyl nitrosourea-induced tumour in a (C57BL/6XDBA/2) F1 mouse. This line is positive for cytoplasmic Ig and negative for surface membrane Ig and TdT.

Nährmedium

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 2-5x100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr P Kincade, Oklahoma Medical Research Foundation