TM3

91060526, mouse testis, Not specified

• UNSPSC-Code: 41106514

Eigenschaften

• product name: TM3, 91060526
• Biologische Quelle: mouse testis
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Prostaglandin F2a
• Rezeptoren: Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse BALB/c testis, Leydig cell

Beschreibung der Zelllinie

Originated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic.

Anwendung

Hormone studies

Nährmedium

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37²C.

Sonstige Hinweise

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