CHP 4 (W.W.)

90102537, human skin, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: CHP 4 (W.W.), 90102537
• Biologische Quelle: human skin
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human skin asymptomatic Galactosemia

Beschreibung der Zelllinie

Derived from skin tissue taken from an 8 year old male with asymptomatic galactosemia, despite lacking red cell galactose-1-phosphate uridyl transferase. Sibling of CHP 3 (M.W.) (Sigma catalogue no. 90102536) with the same enzyme deficiency that was symptomatic. Cultures will retain characteristics for at least 30 passages. Ethnicity: Black.

Anwendung

Enzyme deficiency

Nährmedium

DMEM + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Sonstige Hinweise

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