McCoy

90010305, mouse unknown/unspecified, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: McCoy, 90010305
• Biologische Quelle: mouse unknown/unspecified
• Verpackung: tube of 5 μg 90010305-DNA-5UG; pkg of vial of cells 90010305-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse fibroblast

Beschreibung der Zelllinie

Originally derived from synovial fluid of a patient suffering from degenerative arthritis. However, subsequent sub-lines shown to be of mouse origin, with some characteristics similar to the strain L line. Cells have been used to propagate Chlamydia spp.

Anwendung

Propagation of Chlamydia spp.

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.