HFL1

89071902, human lung (fetal), Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: HFL1, 89071902
• Biologische Quelle: human lung (fetal)
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, diploid
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human foetal lung fibroblast

Beschreibung der Zelllinie

Derived from a 16-18 week old human foetus. Finite lifespan; capable of at least 57 population doublings.

Anwendung

Virus studies

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 7,9
TPOX: 6,9
vWA: 17

Nährmedium

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Sonstige Hinweise

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