HL60 Ast.3

88120801, human blood, Lymphoblastoid

• UNSPSC-Code: 41106514

Eigenschaften

• product name: HL60 Ast.3, 88120801
• Biologische Quelle: human blood
• Wachstumsmodus: Suspension
• Karyotyp: Modal no. = 93, Hypertetraploid
• Morphologie: Lymphoblastoid
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian promyelocytic leukaemia

Beschreibung der Zelllinie

Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Cells are capable of differentiating into neutrophils by induction with 1.75% DMSO.

Anwendung

Differentiation studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 16

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Do not allow the culture medium to become acidic or the cell line will differentiate.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.