KHOS-240S

86112810, human bone, Epithelial

• UNSPSC-Code: 41106514
• Biologische Quelle: human bone
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, aneuploid
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Relevante Krankheit(en): cancer

Eigenschaften

• Produktbezeichnung: KHOS-240S, 86112810
• Biologische Quelle: human bone
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, aneuploid
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian osteosarcoma

Beschreibung der Zelllinie

A revertant line of the KHOS/NP cell line (Sigma Catalogue number. 84102903). Established after overnight incubation at 40.5°C and then sub-cloned at 36°C. The line has similar properties to the parent HOS cell line. Cells are non-tumourigenic in nude mice and no longer possess a rescuable MSV genome.

Anwendung

Tumourigenicity, transformation, biochemical and virus studies: MSV

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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