H6/31HL

85102214, Reactivity: Anti-IgD, mouse

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myelom: P3X63Ag8
• Reaktivität: Anti-IgD, mouse
• Methode(n): cell culture | mammalian: suitable
• Isotyp: IgM
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse P3X63Ag8 x Mouse BALB/c spleen

Beschreibung der Zelllinie

The antibody is directed against ′IgD-like′ molecules of mice with IgB haplotype. The target antigen has a molecular weight of 185kDa and is found on a high proportion of mature lymphoid cells, particularly on cells from Peyer′s patches. It is not detected on thymus cells.

Immunogen

Two grafts of full thickness skin from B10 mice followed by B10 spleen cells

Nährmedium

DMEM + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

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Haftungsausschluss

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.