TA3 Hauschka

85061102, mouse breast, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: TA3 Hauschka, 85061102
• Biologische Quelle: mouse breast
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 41
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse mammary carcinoma

Beschreibung der Zelllinie

In form of tumorigenic ascites shows diminished expression of histocompatibility antigens and readily overcomes histoincompatibility barriers. Immunoresistant TA3/Ha sub-line arose spontaneously without subjection to any known immunological selection pressure.

Anwendung

Tumourigenicity studies

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells grow partly in suspension, therefore spin down supernatant at time of subculture to collect the cells.

Sonstige Hinweise

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