RAMOS Cell Line human

85030802, human lymph, Lymphoblast

• Synonym(e): RA 1 Cells, Ramos (RA 1) Cells, Ramos 1 Cells, Ramos-RA1 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: RAMOS Cell Line human, 85030802
• Biologische Quelle: human lymph
• Beschreibung: Human Caucasian Burkitt′s lymphoma
• Wachstumsmodus: Suspension
• Karyotyp: Modal no. 45
• Morphologie: Lymphoblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian Burkitt′s lymphoma

Beschreibung der Zelllinie

Derived from a Burkitt′s lymphoma which does not possess the EBV genome. EBV infectability and permanent conversion into EBV positive sub-lines is possible by in vitro infection. The cells have B lymphocyte characteristics, with surface associated mu and kappa chains.

Anwendung

Tumourigenicity and virus studies: Epstein-Barr virus (EBV).
RAMOS Cell Line human has been used to carry out a MTT (colorimetric assay) cell viability assay to measure calcium ionophore-induced cell death in Ramos cells under different conditions and to determine the expression of ZFP36 ring finger protein like 1 (ZFP36L1) in Ramos B cells during different stages of B cell differentiation.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 10,13
D5S818: 7,12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

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