11-4

11033102, human cervix, Epithelial

• Synonym(e): 11-4
• UNSPSC-Code: 41106514

Eigenschaften

• product name: 11-4, 11033102
• Biologische Quelle: human cervix
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice

Beschreibung

Ursprung der Zelllinie

HeLa, genetic modification, firefly luciferase reporter gene, HSV-thymidine kinase promoter

Beschreibung der Zelllinie

11-4 is a HeLa cell line stably transfected with a firefly luciferase reporter gene under the control of the HSV-thymidine kinase promoter. 11-4 can be used to evaluate the non-specific transcriptional activity of chemicals.

DNA-Profil

STR-PCR Data:

Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Nährmedium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16 μg/ml Blasticidin S HCL

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Sonstige Hinweise

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Haftungsausschluss

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.