Sf21 TitreHigh AC free

5030202, insect ovary, Spherical

• UNSPSC-Code: 41106514

Eigenschaften

• product name: Sf21 TitreHigh AC free, 05030202
• Biologische Quelle: insect ovary
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Spherical
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Spodoptera frugiperda pupal ovarian tissue, serum-free

Beschreibung der Zelllinie

This cell line is a derivative of the Sf21 cell line which has been adapted to TiterHigh Sf Insect Medium (Sigma I5408). TiterHigh Sf Insect Medium is an animal component-free, protein-free formulation designed specifically for the Sf21 (Spodoptera frugiperda) insect cell line. The cell line Sf21 is derived from pupal ovarian tissue of spodoptera frugiperda. The Sf21 cell line is highly susceptible to Baculovirus infection and is used in the production of protein products genetically manipulated into Baculovirus vector systems.

Anwendung

Protein expression using the Baculovirus vector system, serum-free

Nährmedium

TiterHigh^™ Sf Insect Medium (Sigma I5408) no supplements.

Subkultur-Routine

Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 7.

Sonstige Hinweise

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Rechtliche Hinweise

TiterHigh is a trademark of Sigma-Aldrich Co. LLC