Vero-Hektor

3092503, African green monkey kidney, Fibroblast-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: Vero-Hektor, 03092503, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• Biologische Quelle: African green monkey kidney
• Wachstumsmodus: Adherent
• Karyotyp: Not Specified
• Morphologie: Fibroblast-like
• Rezeptoren: Not specified

Beschreibung

Ursprung der Zelllinie

African green monkey kidney cells, serum-free

Beschreibung der Zelllinie

Vero cell line (Sigma catalog no. 84113001) adapted to grow in the chemically defined protein- and peptide-free medium Hektor G (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent and may be suitable for the replication of viral particles.

Nährmedium

Hektor G medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513). When freezing cells down use Hektor G medium + 4 mM L-Glutamine (G7513) + 10% DMSO (D2650).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm². Use EDTA only (no trypsin); 5% CO₂; 37 °C.

Sonstige Hinweise

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