CHSE-214

21714, fish embryo, Fibroblast-like

• Synonym(e): CHSE/F
• UNSPSC-Code: 41106514

Eigenschaften

• product name: CHSE-214, 00021714
• Biologische Quelle: fish embryo
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Common bluegill (Lepomis macrochirus), normal

Beschreibung der Zelllinie

The fish line CHSE/F was derived from Common bluegill (Lepomis macrochirus) embryo. It is susceptible to various fish viruses including infectious hematopoietic necrosis virus (IHNV); viral haemorrhagic septicaemia virus (VHSV); spring viraemia of carp virus (SVCV); and infectious pancreatic necrosis virus (IPNV).
ECACC has been advised by the CCLV that this cell line is derived from Lepomis macrochirus (Common bluegill) not Oncorhynchus tshawytscha (Chinook salmon) as originally reported. This has been confirmed by DNA sequencing at ECACC. The CCLV have changed the cell line name to CHSE/F to indicate this. The line is used in viral diagnostics and gives better results than BF-2 (Sigma Catalogue number 00021712), a commonly used cell line of Lepomis macrochirus. The sequence of both lines (BF-2 and CHSE/F possess the same nucleotide exchange but are different in culture behaviour and in virus propagation.

Anwendung

Fish virus studies

Nährmedium

EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 120mg/l Sodium Pyruvate(NaP) (S8636) + 10% FBS / FCS (F2442).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; grow cells in dark (e.g. wrapped in foil) at 20-26ºC with 26ºC optimum temperature; monolayer is stable for at least 10 days with maintenance medium containing 1-2% FBS.

Sonstige Hinweise

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