C6788

4-Chlor-1-Naphthol

peroxidase substrate, tablet, suitable for use in immunoblotting procedures

• Synonym(e): 4-CN, 4-chloronaphthalen-1-ol
• Lineare Formel: ClC₁₀H₆OH
• CAS-Nummer: 604-44-4
• Molekulargewicht: 178.61
• Beilstein: 509750
• EG-Nummer: 210-068-5
• MDL-Nummer: MFCD00003974
• UNSPSC-Code: 12352204
• PubChem Substanz-ID: 57654035
• NACRES: NA.83

Eigenschaften

• Produktbezeichnung: 4-Chlor-1-Naphthol, tablet
• Form: tablet
• Qualitätsniveau: 200
• mp (Schmelzpunkt): 118-121 °C (lit.)
• Löslichkeit: methanol: 10 mL, clear, colorless
• Lagertemp.: −20°C
• SMILES String: Oc1ccc(Cl)c2ccccc12
• InChI: 1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H
• InChIKey: LVSPDZAGCBEQAV-UHFFFAOYSA-N

Beschreibung

Spezifität

4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.

Anwendung

4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots. It has also been used as a precipitating chromogenic substrate for visualizing the antigen−antibody-reactive bands following western blotting.

Menge

Contains 30 mg substrate per tablet.

Rekonstituierung

Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves