C3306

Calciumchlorid Dihydrat

for molecular biology, ≥99.0%

• Synonym(e): CaCl₂.2H₂O, Calcium chloride, Calcium dichloride
• Empirische Formel (Hill-System): CaCl₂ · 2H₂O
• CAS-Nummer: 10035-04-8
• Molekulargewicht: 147.01
• EG-Nummer: 233-140-8
• MDL-Nummer: MFCD00149613
• UNSPSC-Code: 12352302
• PubChem Substanz-ID: 24892603
• NACRES: NA.31

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 200
• Assay: ≥99.0%
• Form: powder
• mp (Schmelzpunkt): 176 °C (dec.) (lit.)
• Löslichkeit: H₂O: 294 mg/mL, clear, colorless
• Fremdaktivität: DNase, RNase, protease, none detected
• SMILES String: O.O.Cl[Ca]Cl
• InChI: 1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2
• InChIKey: LLSDKQJKOVVTOJ-UHFFFAOYSA-L

Beschreibung

Anwendung

Calcium chloride dihydrate has been used:
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)

Biochem./physiol. Wirkung

Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H319
• P-Sätze: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Gefahreneinstufungen: Eye Irrit. 2
• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves