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C1261

Sigma-Aldrich

Carboxypeptidase A−Agarose

ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas

Synonym(e):

Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase

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About This Item

EC-Nummer:
3.4.17.1 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00130718
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

enzyme from bovine pancreas

Form

ammonium sulfate suspension

Spezifische Aktivität

≥6 units/mL packed gel, 25 °C

Mol-Gew.

~35,250

Matrix

beaded agarose

Lagertemp.

2-8°C

Allgemeine Beschreibung

Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.

Biochem./physiol. Wirkung

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.
Carboxypeptidase A is attached covalently to agarose or aldehyde and is effective for immobilization studies.

Einheitendefinition

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

Physikalische Form

Suspension in 2.0 M (NH4)2SO4, pH 7

Inhibitor

Produkt-Nr.
Beschreibung
Preisangaben

Substrat

Produkt-Nr.
Beschreibung
Preisangaben

H6875

Hippuryl-Chlor-L-Phenylalanin

Piktogramme

Health hazardExclamation mark
GHS08,GHS07

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Sigma-Aldrich

M6882

Methyl α-D-Mannopyranosid

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Protokolle

Enzymatic Assay of Carboxypeptidase

Objective: To standardize a procedure for the assay of Carboxypeptidase A

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