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Merck

C-12380

Sigma-Aldrich

Human Aortic Adventitial Fibroblasts (HAoAF)

500,000 cryopreserved cells

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About This Item

UNSPSC-Code:
41106514
NACRES:
NA.81

Biologische Quelle

human aorta

Verpackung

pkg of 500,000 cells

Morphologie

(fibroblast)

Methode(n)

cell culture | mammalian: suitable

Relevante Krankheit(en)

hypertension; cardiovascular diseases

Versandbedingung

dry ice

Lagertemp.

−196°C

Allgemeine Beschreibung

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Ursprung der Zelllinie

Aorta

Anwendung

Primary Human Aortic Adventitial Fibroblasts (HAoAF) are isolated from human aortic adventitial tissue and are provided in a cryopreserved format. HAoAF can be used for studies concerning vascular diseases e.g. neointimal formation, vascular remodeling, atherosclerosis, and hypertension. They are also often used for the examination of fibroblast disorders like fibrosis or other diseases linked to either imperfect or excessive accumulation of fibroblasts.

Qualität

Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warnhinweis

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subkultur-Routine

Click here for more information.

Sonstige Hinweise

Recommended Plating Density: 3500 - 7000 cells per cm2Passage After Thawing: P2Tested Markers: CD90 positiveGuaranteed population doublings: >10

Empfohlene Produkte

Recommended Primary Cell Culture Media:Link

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Artikel

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

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