B9285

5-Brom-2′-deoxyuridin

BioUltra, ≥99%

• Synonym(e): 2′-Deoxy-5-bromuridin, 5-BrdU, 5-Brom-1-(2-deoxy-β-D-ribofuranosyl)-uracil, 5-Bromuracil-deoxyribosid, BUdR
• Empirische Formel (Hill-System): C₉H₁₁BrN₂O₅
• CAS-Nummer: 59-14-3
• Molekulargewicht: 307.10
• Beilstein: 30395
• EG-Nummer: 200-415-9
• MDL-Nummer: MFCD00006529
• UNSPSC-Code: 41106305
• PubChem Substanz-ID: 24892132
• NACRES: NA.51

Eigenschaften

• Biologische Quelle: synthetic (organic)
• Produktlinie: BioUltra
• Assay: ≥99%
• Form: powder
• Verunreinigungen: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• Glührückstand: ≤0.1%
• mp (Schmelzpunkt): 191-194 °C (dec.) (lit.)
• Löslichkeit: NH₄OH: 0.1 M at 20 °C, clear, colorless
• Kationenspuren: Al: ≤0.0005%; Ca: ≤0.01%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.05%; Pb: ≤0.001%; Zn: ≤0.0005%
• Lagertemp.: −20°C
• SMILES String: OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
• InChIKey: WOVKYSAHUYNSMH-RRKCRQDMSA-N

Beschreibung

Allgemeine Beschreibung

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light.

Anwendung

5-Bromo-2′-deoxyuridine has been used:

• to label proliferating cells in pregnant mice in cell proliferation assay
• to study the time course of cell proliferation at various times following ischemia, to confirm re-entry of MKI67+ cells in cell cycle to germ cells
• in 5′-Bromo-2′Deoxyuridine (BrdUrd) staining of fibroblasts

Biochem./physiol. Wirkung

5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.

5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.

Thymidin-Analogon zur Verwendung als Mutagen in der Genforschung. Selektiver Einbau in zelluläre DNA während der S-Phase.

Sonstige Hinweise

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H340,H361fd
• P-Sätze: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Gefahreneinstufungen: Muta. 1B - Repr. 2
• Lagerklassenschlüssel: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)