A7554

Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse

clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution

• Synonym(e): Monoclonal Anti-Bovine IgG
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: alkaline phosphatase conjugate
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: secondary antibodies
• Klon: BG-18, monoclonal
• Form: buffered aqueous glycerol solution
• Speziesreaktivität: bovine (non-reactive to IgM)
• Darf nicht reagieren mit: pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat
• Methode(n): direct ELISA: 1:25,000; dot blot: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG; western blot (chemiluminescent): 1:100,000
• Isotyp: IgG1
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.

Spezifität

bovine IgG1 and IgG2

Immunogen

Purified bovine IgG

Anwendung

Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in:

• western blotting
• enzyme linked immunosorbent assay (ELISA)
• dot blot
• immunohistochemistry

Biochem./physiol. Wirkung

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Physikalische Form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)