A6338

Aldehyd-Dehydrogenase, Kalium-aktiviert aus Backhefe (S. cerevisiae)

lyophilized powder, ≥2.0 units/mg protein

• Synonym(e): Aldehyd:NAD[P]⁺-Oxidoreduktase
• CAS-Nummer: 9028-88-0
• EC-Nummer: 1.2.1.5 (BRENDA, IUBMB)
• EG-Nummer: 232-846-3
• MDL-Nummer: MFCD00081306
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Form: lyophilized powder
• Qualitätsniveau: 200
• Spezifische Aktivität: ≥2.0 units/mg protein
• Mol-Gew.: 228 kDa
• Zusammensetzung: Protein, ≥5.0% biuret
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Aldehyde dehydrogenase is a tetramer and has several different isoforms. The enzyme tested in 0.01 M pyrophosphate buffer shows a sharp optimum around pH 9.3 with acetaldehyde as substrate. Potassium ions and cysteine are essential for the enzyme′s activity. Rubidium or NH4⁺ can be substituted for K⁺, and glutathione for cysteine. Lithium, Na⁺, and Cs⁺ inhibit the reaction. Aldehyde dehydrogenase is inhibited by propylurea, crotonaldehyde, n-propyl isocyanate, cyclohexyl isocyanate, 1-n-propyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea, and 1-methyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea. The enzyme may be utilized to quantitate aldehydes present in blood.

Aldehyde dehydrogenase (ALDH) is present in the nucleus, cytosol, mitochondria and endoplasmic reticulum of cells.

Anwendung

Aldehyde dehydrogenase (ALDH) has been used to evaluate the effects of pear extracts on ALDH activity. It has also been used to colorimetrically determine ethanol by monitoring the enzymatic reduction of nicotinamide adenine dinucleotide (NAD).

Biochem./physiol. Wirkung

Aldehyde dehydrogenase from baker′s yeast catalyzes the reduction of pyridine nucleotides by several aldehydes. It catalyzes the oxidation of a wide range of substrates, such as acetaldehyde, formaldehyde, propionaldehyde, n-butylaldehyde, isobutylaldehyde, n-valeraldehyde, caproaldehyde, benzaldehyde, glycoaldehyde, D-glyceraldehyde, malonic semialdehyde, and succinic aldehyde. Aldehyde dehydrogenase is used to study the production of ethanol and isobutanol. Ethanol concentration can be determined colorimentrically by monitoring the enzymatic reduction of nicotinamide adenine dinucleotide (NAD) using alcohol dehydrogenase after preremoval of aldehyde by aldehyde dehydrogenase.

Einheitendefinition

1 U oxidiert 1.0 μmol Acetaldehyd zu Essigsäure pro Minute bei 25 °C und pH 8.0 in Anwesenheit von β-NAD⁺, Kalium und Thiolen.

Physikalische Form

Enthält Trehalose, Kaliumphosphat und Citrat-Puffersalze, Dithiothreitol und Spuren von β-NAD und Propionsäure.

Rekonstituierung

This enzyme can be dissolved at 0.3 mg/mL in 100 mM Tris-HCl buffer (pH 8.0), containing 0.02% BSA.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)