A5463

Anti-Acetylated Proteins antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• MDL-Nummer: MFCD03454689
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Speziesreaktivität: human, chicken, bovine
• Konzentration: ~0.5 mg/mL
• Methode(n): indirect immunofluorescence: 1:1,000 using methanol/acetone-fixed chicken fibroblats cells; microarray: suitable; western blot: 1:1,000 using Whole cell extract of human epitheloid carcinoma HeLa cell line treated with sodium butyrate
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Protein acetylation on lysine is a reversible protein modification that regulates protein functions. Histone acetylation at N-epsilon-lysine residues can destabilize the nucleosome structure and render DNA more accessible to transcription factors. Protein acetylation controls the function of transcription factors such as p53, GATA-1, E2F, TFIIEb, TFIIF, and EKLF.
Staining of histone H4 in immunoblotting is specifically inhibited with acetylated histone H4 immunizing peptide (human, amino acids 1-20 with N-epsilon-acetylated lysines at positions 5, 8, 12, and 16). No inhibition with the non-acetylated histone H4 peptide (human, amino acids 1-20) is observed.

Reacts with several acetylated proteins (e.g., acetylated histone H4 [12 kDa], acetylated H1 and H2b and acetylated BSA). Does not recognize acetylated histone H3.

Immunogen

synthetic acetylated peptide corresponding to the N-terminus of human histone H4 (amino acids 1-20, with N-ε-acetylated lysines at positions 5,8,12 and 16) conjugated to KLH.

Anwendung

Rabbit anti-acetylated protein antibody can be used for microarrays, indirect immunofluorescence (1:1,000) and western blot (1:1,000) assays.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable