A3151
Avidin-Peroxidase aus Hühnereiweiss/Meerrettich
lyophilized powder
Synonym(e):
Avidin-POD
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About This Item
Empfohlene Produkte
Biologische Quelle
avidin from egg white
enzyme from horseradish
Qualitätsniveau
Konjugat
peroxidase conjugate
Form
lyophilized powder
Zusammensetzung
Protein, ≥70% E1%/280
Kennzeichnungsgrad
0.7-2.0 mol peroxidase per mol avidin
Methode(n)
direct ELISA: 1:50,000
Löslichkeit
H2O: soluble 1 mg/mL
PBS: soluble
Lagertemp.
−20°C
Allgemeine Beschreibung
Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Anwendung
Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding and OVA-IgG1.
To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.
Physikalische Form
Lyophilized powder containing citrate buffer.
Angaben zur Herstellung
Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signalwort
Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Analysenzertifikate (COA)
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