A2580

Angiotensin Converting Enzyme from porcine kidney

lyophilized powder, ≥10 units/mg protein (Bradford)

• Synonym(e): Peptidyl-Dipeptidase A
• CAS-Nummer: 9015-82-1
• EC-Nummer: 3.4.15.1 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00130325
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Form: lyophilized powder
• Qualitätsniveau: 200
• Spezifische Aktivität: ≥10 units/mg protein (Bradford)
• UniProt-Hinterlegungsnummer: A0A480IQM9
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Angaben zum Gen: pig ... ACE(613133)

Beschreibung

Allgemeine Beschreibung

ACE is a monomer with molecular weight of ~170 kDa
pH range for activity: 7-8.5
Temperature optimum: 37 °C
Zinc is required for activity
Inhibitors: captopril, enalaprilat, lisinopril etc. (1-10 μM)

Angiotensin converting enzyme (ACE) is encoded by the gene, mRNA-decapping enzyme subunit 1 (DCP1). It is a zinc metalloprotease, which belongs to the M2 family.

Anwendung

Angiotensin converting enzyme from porcine kidney has been used in in vitro angiotensin-converting enzyme (ACE) inhibition assay.

Biochem./physiol. Wirkung

Angiotensin converting enzyme (ACE) catalyzes the conversion of angiotensin I to angiotensin II, which regulates the fluid-electrolyte balance and systemic blood pressure. ACE inhibits the vasodilator, bradykinin. ACE inhibitors are used to treat high blood pressure.

Removes C-terminal dipeptides from susceptible substrates, e.g., angiotensin I and bradykinin.

Einheitendefinition

One unit will produce 1.0 ·μmole of hippuric acid from Hippuryl-His-Leu per min in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 °C.

Physikalische Form

Lyophilized powder containing Tris buffer salts.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)