99072804

T-3-CI-2-0.fl cell line

mouse spleen, Lymphoblastoid-like

• UNSPSC-Code: 41106514
• NACRES: NA.81

Eigenschaften

• product name: T-3-CI-2-0.fl cell line,
• Biologische Quelle: mouse spleen
• Qualitätsniveau: 100
• Form: liquid
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblastoid-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse friend leukemia lymphoblast

Beschreibung der Zelllinie

T-3-Cl-2-0.fl is a clone of T-3-Cl-2, which originated from a splenic lesion of Friend virus-induced leukemia in a DD mouse. The cell line can be induced to differentiate into erythroblastoid cells with 1.5% DMSO. T-3-Cl-2-0.fl has been eradicated from mycoplasma using MC-210 by the originator.

Anwendung

Study of erythroid differentiation

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5%CO₂; 37ºC

Sonstige Hinweise

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Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable