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94060901

FTC-133 Cell Line human

Synonym(e):

FTC133 Cells

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About This Item

UNSPSC-Code:
41106514

Biologische Quelle

human thyroid (lymph node metastasis)

Beschreibung

Human follicular thyroid carcinoma

Form

liquid

Wachstumsmodus

Adherent

Karyotyp

Complex chromosomal changes

Morphologie

Polymorphic

Produkte

Expression of 5′ - Deiodinase Type I

Rezeptoren

Not specified

Methode(n)

cell culture | mammalian: suitable

Relevante Krankheit(en)

metastasis

Versandbedingung

dry ice

Lagertemp.

−196°C

Ursprung der Zelllinie

Human follicular thyroid carcinoma, lymph node metastasis

Beschreibung der Zelllinie

FTC-133 was obtained from a lymph node metastasis of a follicular thyroid carcinoma not the primary tumour as originally reported from a 42-year-old male. The morphology differs from flat polygonal to spindle formed cells. They retained differentiated thyrocyte function and thyrocyte responsiveness to thyrotropin and local active growth factors. Thyroglobulin immunoreactivity could be shown in the cytoplasm and EGF receptor immunoreactivity could be visualised on the cell membranes. Complex chromosomal changes were detected as well as a p53 mutation. FTC-133 (Sigma catalogue number 94060901), FTC-236 (Sigma catalogue number 06030202) and FTC-238 (Sigma catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.

Anwendung

FTC-133 cell line has been used to study the function of human thyroid and development of thyroid cancer.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 9,10
THO1: 9.3
TPOX: 9
vWA: 15,18

Nährmedium

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:8 to 1:12 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

C Hoang-Vu et al.
Laboratory animal science, 42(2), 164-167 (1992-04-01)
The thyroid physiology of athymic nude rats, rnu/rnu, is characterized and established here as an animal model to study transplanted thyroid tumors. Male rats were catheterized 5 days before experiments were started. The mean thyroid-stimulating-hormone (TSH) plasma concentrations were 2.9
M J Demeure et al.
Surgery, 108(6), 986-992 (1990-12-01)
The actin cytoskeleton is important for cell structure and motility. A disordered actin architecture has been correlated with a high metastatic potential in melanoma, fibrosarcoma, and colon cancer models. Thyrotropin is known to induce growth and differentiation in cultured thyroid
T Hölting et al.
European journal of endocrinology, 132(2), 229-235 (1995-02-01)
We have shown recently that epidermal growth factor (EGF) enhanced invasion and growth of differentiated thyroid cancer cells in vitro and in vivo. The present study analyzed the effects of transforming growth factor alpha (TGF-alpha) on invasion and growth of
T Hoelting et al.
Biochemical and biophysical research communications, 195(3), 1230-1236 (1993-09-30)
The signal transduction of TSH in invasion and growth of FTC 133, a human follicular thyroid cancer cell line, was investigated. TSH (0.01-1 mIU/ml) stimulated invasion of FTC 133 by 21% and growth by 20% of basal. Cyclic AMP-stimulators and
Hypermethylation of a New Distal Sodium/Iodide Symporter (NIS) Enhancer (NDE) Is Associated With Reduced NIS Expression in Thyroid Tumors
Ana Luiza Galrao
The Journal of Clinical Endocrinology and Metabolism, 99(6) (2014)

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