73737

4-Nitrophenylphosphat Bis-(Tris)-salz

phosphatase substrate, ≥97.0% (enzymatic), powder or crystals

• Synonym(e): p-Nitrophenylphosphate bis(tris) salt, p-nitrophenyl phosphate bis(tris) salt, PNPP, PNPP bis(tris) salt
• Lineare Formel: (C₆ H₄ N O₆ P)( C₄ H₁₂ N O₃)₂
• CAS-Nummer: 68189-42-4
• Molekulargewicht: 461.36
• Beilstein: 1578142
• EG-Nummer: 269-198-6
• MDL-Nummer: MFCD00037009
• UNSPSC-Code: 12352204
• PubChem Substanz-ID: 57652488
• NACRES: NA.32

Eigenschaften

• product name: 4-Nitrophenylphosphat Bis-(Tris)-salz, for the determination of phosphatase, ≥97.0% (enzymatic)
• Assay: ≥97.0% (enzymatic)
• Form: powder or crystals
• Qualität: for the determination of phosphatase
• Verunreinigungen: ≤0.1% free nitrophenol; ≤6% water
• Löslichkeit: H₂O: 0.1 g/mL, clear
• Lagertemp.: 2-8°C
• SMILES String: NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
• InChIKey: XXAXKCWOTRABOW-UHFFFAOYSA-N

Beschreibung

Anwendung

4-Nitrophenyl phosphate bis(tris) salt has been used to determine the enzyme activity of acid and alkaline phosphatase in cell lysates.

Biochem./physiol. Wirkung

4-Nitrophenyl phosphate is a non-proteinaceous and non-specific chromogenic substrate, preferred for alkaline phosphatase (ALP) in enzyme-linked immunosorbent assay (ELISA). It is widely used to assay protein tyrosine phosphatases (PTPs). Upon cleavage, the substrate produces a yellow product that is spectrophotometrically measured.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)