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5006

Sigma-Aldrich

Bovine Collagen Type I & III

from bovine hide, powder, suitable for cell culture, PureCol™

Synonym(e):

Collagen Type I, PureCol Collagen

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About This Item

EG-Nummer:
232-697-4
UNSPSC-Code:
12352202
NACRES:
NA.75

product name

PureCol™, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture

Qualitätsniveau

100

Sterilität

sterile-filtered

Assay

99.9%

Form

lyophilized

Verpackung

pkg of 15 mg

Methode(n)

cell culture | mammalian: suitable

Bindungsspezifität

Peptide Source: Elastin

Peptide Source: Fibrinogen

Versandbedingung

wet ice

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

PureCol™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Anwendung

PureCol™ has been used:
  • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
  • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
  • to coat culture plates for collagen degradation assay with breast cancer cell lines

Angaben zur Herstellung

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Rechtliche Hinweise

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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