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Merck

18507

Sigma-Aldrich

Atto 565 Maleimid

BioReagent, suitable for fluorescence

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About This Item

Empirische Formel (Hill-System):
C37H37ClN4O10
Molekulargewicht:
733.16
MDL-Nummer:
UNSPSC-Code:
12352111
NACRES:
NA.32

Produktlinie

BioReagent

Qualitätsniveau

Assay

>90.0% (HPLC)

Form

powder

Hersteller/Markenname

ATTO-TEC GmbH

Durchlässigkeit

254 nm
565 nm

Fluoreszenz

λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0

λ

(ethanol with 0.1% trifluoroacetic acid)

UV-Absorption

λ: 560-566 nm Amax

Eignung

corresponds for coupling to thiols
suitable for fluorescence

Lagertemp.

−20°C

Anwendung

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

Rechtliche Hinweise

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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