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14340C

SAFC

EX-CELL® 325 PF CHO Serum-Free Medium for CHO Cells, Protein Free

protein free, liquid, sterile-filtered, suitable for cell culture

Arzneimittelherstellung

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About This Item

MDL-Nummer:
MFCD01634638
UNSPSC-Code:
12352207
NACRES:
NA.75

Beschreibung

for research or for further manufacturing use
without L-glutamine

Qualitätsniveau

400

Sterilität

sterile-filtered

Form

liquid

Methode(n)

cell culture | mammalian: suitable

Komponenten

glucose: 3.45 g/L
L-glutamine: no
NaHCO3: 1.6 g/L
HEPES: 7.5 mM
phenol red: yes

Versandbedingung

ambient

Lagertemp.

2-8°C

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Anwendung

EX-CELL™ 325 PF CHO is a protein-free medium which has been developed for the growth of Chinese Hamster Ovary (CHO) cells. Because it contains no large macromolecules, EX-CELL 325 PF CHO facilitates the isolation and purification of secreted proteins from the cells. CHO cells propagated in EX-CELL 302 serum-free media (Catalog No. 14324C and 14326C) can be transferred directly into this protein-free medium without extensive weaning protocols. This medium is supplied without L-glutamine and does not contain purines or pyrimidines to provide an appropriate medium for specialized CHO cell lines (i.e., Glutamine Synthetase, or the GS System™, and DHFR- selection systems).

Rechtliche Hinweise

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Ala-Gln 200 mM, solution, sterile-filtered, Biotechnology Performance Certified

Sigma-Aldrich

G8541

Ala-Gln

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Sapan J Patel et al.
Electrophoresis, 38(3-4), 417-428 (2016-11-03)
The mechanisms underlying the aberrant growth and interactions between cells are not understood very well. The pre-B acute lymphoblastic leukemia cells directly obtained from an adult patient grow very poorly or do not grow at all at low density (LD)

Artikel

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

Selection Process Development in a GS Knock-Out CHO Host Cell Line: The Effects of MSX Addition on Clones Generated in an MSX-Free Process
Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

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