PPB019

Bis-Tris Bicine EDTA buffer

pH 7.2, pHast Pack^™, powder

• Synonym(e): 1X Bis-Tris transfer buffer, Bis-Tris
• UNSPSC-Code: 41105319
• NACRES: NA.25

Eigenschaften

• Produktlinie: pHast Pack^™
• Qualitätsniveau: 100
• Form: powder
• Lagerbedingungen: (Tightly closed. Dry)
• pH-Wert: 7.2
• Kationenspuren: Pb: ≤5 ppm
• Fremdaktivität: DNAse, free; NICKase, free; Protease, free; RNAse, free
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

Bis-Tris Bicine EDTA buffer or Bis-Tris buffer (pH 7.2) contains Bis-Tris and Bicine, zwitterionic buffer components that form strong bonds with common metals. In combination with ethylenediaminetetraacetic acid (EDTA), a metal chelator, that binds divalent cations. This buffer is commonly used as a transfer buffer in western blot to transfer proteins from the gel to the membrane.

Anwendung

Bis-Tris buffer may be used in western blot as a transfer buffer.

Leistungsmerkmale und Vorteile

• Ready-to-use buffer
• No measuring and pH adjustment needed
• Certified quality for reproducibility
• Minimizes exposure to toxic chemicals
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Verpackung

Foil pouches

Rekonstituierung

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1X solution containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, pH 7.2 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Lagerung und Haltbarkeit

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Sonstige Hinweise

For additional information on our range of Biochemicals, please complete this form.

Rechtliche Hinweise

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Danger
• H-Sätze: H318
• P-Sätze: P280 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Dam. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3