PPB005

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween^® 20, pHast Pack^™

• Synonym(e): PBS + 0.05% TWEEN^® 20, PBS with 0.05% Tween 20, PBS-T, PBST
• UNSPSC-Code: 12161700
• NACRES: NA.21

Eigenschaften

• product name: Phosphatgepufferte Kochsalzlösung mit 0,05 % TWEEN^® 20, pH 7,4, pHast Pack^™, powder
• Produktlinie: pHast Pack^™
• Form: powder
• Lagerbedingungen: (Tightly closed. Dry)
• Verunreinigungen: ≤5 ppm Heavy Metals (lead)
• Farbe: white
• pH-Wert: 7.2—7.6 (25 °C)
• Löslichkeit: soluble, clear to hazy, colorless
• Kationenspuren: Fe: <10 ppm
• Fremdaktivität: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

Phosphate Buffered Saline with 0.05% Tween^® 20 or PBST contains PBS buffer with a low-concentration of Tween^® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

Anwendung

PBST is used in research in a variety of assays including DNA or RNA hybridization assays and cell culture. It is commonly used for immunoassays like Western blot, ELISA, Dot blot, Immunohistochemistry (IHC), immunopercipitation (IP), and immunofluorecence. It has been used for incubation and wash buffer for peptide microarrays for performing High-density peptide array (HDPA) to analyze antibody responses against SARS-CoV-2.

Leistungsmerkmale und Vorteile

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Verpackung

Foil pouches

Rekonstituierung

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Lagerung und Haltbarkeit

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Sonstige Hinweise

For additional information on our range of Biochemicals, please complete this form.

Rechtliche Hinweise

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable